Journal:

Article Title: Cell-wall determinants of the bactericidal action of group IIA phospholipase A 2 against Gram-positive bacteria

doi:

Figure Lengend Snippet: Cell-wall active antibiotics can act synergistically or additively with PLA2 to kill E. faecium. E. faecium strains 4 (a–c) and 6 (d–f) (105/ml) were treated with ampicillin (16 μg/ml; a and d), cephalothin (32 μg/ml; b and e),vancomycin (30 μg/ml; c and f), or no antibiotic for 30 mi n in PHS buffered with 10 mM HEPES (pH 7.4) before treatment with 50 ng/ml (strain 4) or 500 ng/ml (strain 6) of purified rabbit AF group IIA PLA2 at 37°C. At the indicated times, samples were removed to measure viability as described in Methods. The results represent the mean ± SEM of four experiments. Some error bars are masked by the symbols. (open squares) Bacteria alone; (closed squares) antibiotic + bacteria; (open circles) bacteria + PLA2; (closed circles) antibiotic + bacteria + PLA2.

Article Snippet: S. aureus strain 18 and Enterococcus faecium strains 4 and 6 are multi-drug–resistant clinical isolates obtained from P. Tierno and K. Inglima (Department of Clinical Microbiology, Tisch Hospital, New York, New York, USA).

Techniques: Purification, Bacteria